Teenage Mutant Ninja Turtles: Sewer Run is a browser-based action game. The events take place directly after Teenage Mutant Ninja Turtles: Dark Horizons.

The game consists of four levels that each have a racing section and a fighting section. The racing section has the Turtle selected race to catch up with the target before time runs out, while the fighting section has the Turtle defeat a selected number of Foot Soldiers by hitting back shuriken thrown at him.

Every Turtle has their own special move (Leonardo can use his katana to break rocks, Donatello can use his staff to double-jump, Michelangelo can use his kusarigama as a grappling hook to swing around, and Raphael can use his sai to climb up walls). Each Turtle can only be used in one level, meaning that each of the four levels have to be beaten by one Turtle.