Set off on a grand adventure in a fantasy world with the latest entry in the Rune Factory simulation RPG series. After losing their memory, the hero lands in a small town blessed by nature. There, they are recruited into a band of peacekeeping rangers, and their new life begins. In addition to their normal duties, the hero can farm the land, cast a line into the nearby river, and so much more! Team up with townsfolk to battle monsters and unleash powerful combination attacks - just one of the features new to the series. The stage is set, and the curtain rises on an exciting new adventure in Rune Factory 5!

In the Kingdom of Norad, a girl wanders in the forest by herself. She is nearly attacked by a Wooly and screams. Lying on the ground nearby, the player wakes up without memories but realizes they to help the girl. They defeat the monsters and are escorted back to the girl's town, but collapses at the gate. While attempting to take a step out of town, Priscilla and Lucy witness this and rush over to help.

The player wakes up at the clinic and meets Simone and Murakumo, revealing to both of them that they suffer from amnesia. Lucy is not sure if they should allow the player to stay, but her mother suggests they try the Soul Sphere. She goes to Seed Station and introduce them to Livia. As the player touches the Soul Sphere, it shines brightly, surprising everyone. Livia says that the player is not dangerous.