Rail Chase 2 is a 3D rail shooter played from a first-person perspective. It is the sequel to Rail Chase. An organization called the EKKL Empire has abducted the female scientist Mary Bates and forces her to develop weapons for them. Journalist Flint Rickman manages to infiltrate the organization to rescue her. The game starts with the two trying to escape in a rail cart.

While following the rail tracks you have to gun down various enemies soldiers in rail carts, soldiers standing on the side of the track, vehicles and bosses. Occasionally you come across med packs on the side of the rails that can be shot to regain health. Different routes can be taken to escape. The game can be played solo or with a friend.