Battle Athletess Daiundokai GTO (also known as Battle Athletess Daiundokai Grand trial Onnonoko) is the second game based on an anime series: This time the player will compete in a full 3d action obstacle race contest against different rivals in different places.

Battle Athletess is a 6 episode OVA set in the distant future where, after many years of war with an extraterrestrial race, a contest of physical strength between one representative from each race is agreed. Although the alien race is far superior to the humans, the human champion prevailed, marking an era of peace for humanity. A University Satellite is created where the greatest athletes of the age train with women competing for the title of Cosmic Beauty.

Akari Kanzaki, daughter of a former Cosmic Beauty, arrives at the University Satellite excited and ready to compete. After watching defending Cosmic Beauty Lahrri run faster than she ever has despite the artificial gravity being turned up, Akari realizes just how far she needs to go. Helping her are her fellow team members and roommates Kris Christopher, a soon-to-be priestess from the moon, and Anna Respighi, a shy girl with a secret so hidden even she is unaware of it. Kris is marked as a standout athlete and competes on a level near Lahrri drawing the wrath of another top competitor Mylandah onto the team as they prepare for the final competition.